稳定同位素稀释质谱法用于膜转运体定量。
Stable isotope dilution mass spectrometry for membrane transporter quantitation.
出版信息
AAPS J. 2013 Oct;15(4):1222-31. doi: 10.1208/s12248-013-9529-8.
Abstract
This review provides an introduction to stable isotope dilution mass spectrometry (MS) and its emerging applications in the analysis of membrane transporter proteins. Various approaches and application examples, for the generation and use of quantitation reference standards—either stable isotope-labeled peptides or proteins—are discussed as they apply to the MS quantitation of membrane proteins. Technological considerations for the sample preparation of membrane transporter proteins are also presented.
摘要
本文综述了稳定同位素稀释质谱（MS）及其在分析膜转运蛋白中的应用。讨论了各种方法和应用实例，包括用于生成和使用定量参考标准（稳定同位素标记的肽或蛋白质）的方法，这些方法适用于膜蛋白的 MS 定量。还介绍了膜转运蛋白样品制备的技术考虑因素。
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