Nicholson Tristan M, Uchtmann Kristen S, Valdez Conrad D, Theberge Ashleigh B, Miralem Tihomir, Ricke William A
Department of Urology, University of Wisconsin-Madison.
J Vis Exp. 2013 Aug 28(78):50574. doi: 10.3791/50574.
New therapies for two common prostate diseases, prostate cancer (PrCa) and benign prostatic hyperplasia (BPH), depend critically on experiments evaluating their hormonal regulation. Sex steroid hormones (notably androgens and estrogens) are important in PrCa and BPH; we probe their respective roles in inducing prostate growth and carcinogenesis in mice with experiments using compressed hormone pellets. Hormone and/or drug pellets are easily manufactured with a pellet press, and surgically implanted into the subcutaneous tissue of the male mouse host. We also describe a protocol for the evaluation of hormonal carcinogenesis by combining subcutaneous hormone pellet implantation with xenografting of prostate cell recombinants under the renal capsule of immunocompromised mice. Moreover, subcutaneous hormone pellet implantation, in combination with renal capsule xenografting of BPH tissue, is useful to better understand hormonal regulation of benign prostate growth, and to test new therapies targeting sex steroid hormone pathways.
两种常见前列腺疾病，即前列腺癌（PrCa）和良性前列腺增生（BPH）的新疗法，在很大程度上依赖于评估其激素调节的实验。性类固醇激素（特别是雄激素和雌激素）在前列腺癌和良性前列腺增生中起着重要作用；我们通过使用压缩激素丸剂的实验来探究它们在诱导小鼠前列腺生长和致癌过程中的各自作用。激素和/或药物丸剂很容易用丸剂压制机制造，并通过手术植入雄性小鼠宿主的皮下组织。我们还描述了一种通过将皮下激素丸剂植入与免疫缺陷小鼠肾包膜下前列腺细胞重组体的异种移植相结合来评估激素致癌作用的方案。此外，皮下激素丸剂植入与良性前列腺增生组织的肾包膜异种移植相结合，有助于更好地理解良性前列腺生长的激素调节，并测试针对性类固醇激素途径的新疗法。
