Unit of Rural Biotechnology, Saraswathi Narayanan College, Madurai 625022, India.
Biochem Biophys Res Commun. 2013 Oct 4;439(4):539-46. doi: 10.1016/j.bbrc.2013.09.007. Epub 2013 Sep 8.
Stomach lineage specific gene products act as a protective barrier in the normal stomach and their expression maintains the normal physiological processes, cellular integrity and morphology of the gastric wall. However, the regulation of stomach lineage specific genes in gastric cancer (GC) is far less clear. In the present study, we sought to investigate the role and regulation of stomach lineage specific gene set (SLSGS) in GC. SLSGS was identified by comparing the mRNA expression profiles of normal stomach tissue with other organ tissue. The obtained SLSGS was found to be under expressed in gastric tumors. Functional annotation analysis revealed that the SLSGS was enriched for digestive function and gastric epithelial maintenance. Employing a single sample prediction method across GC mRNA expression profiles identified the under expression of SLSGS in proliferative type and invasive type gastric tumors compared to the metabolic type gastric tumors. Integrative pathway activation prediction analysis revealed a close association between estrogen-α signaling and SLSGS expression pattern in GC. Elevated expression of SLSGS in GC is associated with an overall increase in the survival of GC patients. In conclusion, our results highlight that estrogen mediated regulation of SLSGS in gastric tumor is a molecular predictor of metabolic type GC and prognostic factor in GC.
胃系特异性基因产物在正常胃中充当保护屏障，其表达维持胃壁的正常生理过程、细胞完整性和形态。然而，胃癌（GC）中胃系特异性基因的调控远不明确。在本研究中，我们旨在研究胃系特异性基因集（SLSGS）在 GC 中的作用和调控。通过比较正常胃组织与其他器官组织的 mRNA 表达谱来鉴定 SLSGS。结果发现 SLSGS 在胃肿瘤中表达下调。功能注释分析表明，SLSGS 富集了消化功能和胃上皮维持。在 GC mRNA 表达谱中采用单个样本预测方法，发现与代谢型 GC 相比，增殖型和侵袭型 GC 肿瘤中 SLSGS 的表达下调。整合通路激活预测分析显示，GC 中雌激素-α信号与 SLSGS 表达模式密切相关。GC 中 SLSGS 的高表达与 GC 患者总生存率的提高相关。总之，我们的结果强调了雌激素介导的 SLSGS 在胃肿瘤中的调控是代谢型 GC 的分子预测因子和 GC 的预后因素。
