Chappard D, Rossi J F, Bataillle R, Alexandre C
LBTO, Laboratoire de Biologie du Tissu Osseux, Faculté de Médecine, Saint Etienne, France.
Scanning Microsc. 1990 Jun;4(2):323-8.
Tartrate resistant acid phosphatase (TRAP) is a reliable histochemical marker of osteoclasts when used on tissue sections of undecalcified bone. This paper presents an original morphometric analysis which can be done after histochemical identification of osteoclasts. These bone resorbing cells were demonstrated on undecalcified bone biopsies from control subjects and patients presenting a malignant disease of the lymphocyte B lineage. Computerized analysis of the osteoclastic population revealed that: (1) all TRAP positive cells along bone trabeculae belong to a osteoclastic population; (2) that B cell malignancies had an increased bone resorption. At the scanning electron microscopic level small resorption bays (about 10 microns in diameter) were observed either associated or separated from eroded surfaces presenting abnormal appearance; TRAP staining of histological sections of undecalcified bone, coupled with morphometric studies, may help in the understanding of bone disease pathobiology.
抗酒石酸酸性磷酸酶（TRAP）用于未脱钙骨组织切片时，是破骨细胞可靠的组织化学标记物。本文介绍了一种在破骨细胞进行组织化学鉴定后可开展的原创性形态计量分析。在来自对照受试者以及患有B淋巴细胞系恶性疾病患者的未脱钙骨活检组织上证实了这些骨吸收细胞。对破骨细胞群体的计算机分析显示：（1）沿骨小梁的所有TRAP阳性细胞均属于破骨细胞群体；（2）B细胞恶性肿瘤的骨吸收增加。在扫描电子显微镜水平上，观察到直径约10微米的小吸收凹，它们与外观异常的侵蚀表面相关或分离；未脱钙骨组织学切片的TRAP染色，结合形态计量学研究，可能有助于理解骨疾病的病理生物学。
