Laboratories for Biomembrane Research and Biotechnology, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan 200284, Nigeria.
Mol Med Rep. 2013 Nov;8(5):1493-8. doi: 10.3892/mmr.2013.1674. Epub 2013 Sep 9.
The antioxidant properties of robustaside B and para‑hydroxyphenol isolated from Cnestis ferruginea were measured as the rate of inhibition of thiobarbituric acid reactive substance (TBARS) production in the Fe2+/ascorbate system. The modulatory effects of the compounds on mitochondrial membrane permeability transition (MMPT) were monitored spectrophotometrically as decreases in light scattering at 540 nm. The varying concentrations of robustaside B and para‑hydroxyphenol (0.05, 0.1, 0.2, 0.25, 0.5, 0.75 and 1 mM) significantly reduced (P<0.05) the amount of TBARS generated by the Fe2+/ascorbate system by 85.3, 86.4, 86.0, 86.1, 86.0, 86.0 and 86.0% and 86.7, 81.3, 81.3, 80, 80, 82.6 and 83.1%, respectively. Similarly, quercetin, a standard antioxidant, was found to induce an 80% reduction in the amount of TBARS produced. The same IC50 value of 0.025 mM was observed for robustaside B, para‑hydroxyphenol and quercetin. Pre‑incubation of varying concentrations of robustaside B (0.125, 0.2, 0.5 and 1 mM) with succinate‑energized mitochondria induced MMPT pore opening by 0, ‑33.3, ‑59.3 and ‑218.5%, compared with control mitochondria. Para‑hydroxyphenol at 0.1, 0.2, 0.25 and 0.5 mM induced MMPT pore opening in a concentration‑dependent manner up to 0.25 mM by ‑21, ‑54.4 and ‑107.0%, respectively. Quercetin at 0.05, 0.1, 0.25, 0.5, 0.75 and 1 mM also induced MMPT pore opening in the absence of calcium in a concentration‑dependent manner by 5, 3.7, ‑42.6, ‑81.5, ‑187 and ‑161.1%, respectively. The current observations confirm the antioxidant properties of robustaside B and para‑hydroxyphenol, and indicate a potential therapeutic use of the compounds for the treatment of diseases requiring the induction of cell death, including cancer.
从铁青树中分离得到的强啡肽 B 和对羟基苯酚的抗氧化性能，是通过抑制 Fe2+/抗坏血酸体系中硫代巴比妥酸反应物质（TBARS）的产生来衡量的。通过监测 540nm 处光散射的减少，来监测化合物对线粒体膜通透性转换（MMPT）的调节作用。不同浓度的强啡肽 B 和对羟基苯酚（0.05、0.1、0.2、0.25、0.5、0.75 和 1mM）分别显著降低（P<0.05）Fe2+/抗坏血酸系统生成的 TBARS 量 85.3%、86.4%、86.0%、86.1%、86.0%、86.0%和 86.0%和 86.7%、81.3%、81.3%、80%、80%、82.6%和 83.1%。同样，作为标准抗氧化剂的槲皮素被发现可使 TBARS 的产生量减少 80%。强啡肽 B、对羟基苯酚和槲皮素的 IC50 值相同，均为 0.025mM。不同浓度的强啡肽 B（0.125、0.2、0.5 和 1mM）与琥珀酸供能的线粒体预孵育，与对照线粒体相比，诱导 MMPT 孔开放 0%、-33.3%、-59.3%和-218.5%。对羟基苯酚在 0.1、0.2、0.25 和 0.5mM 浓度下，以浓度依赖的方式诱导 MMPT 孔开放，最高可达-21%、-54.4%和-107.0%。槲皮素在 0.05、0.1、0.25、0.5、0.75 和 1mM 浓度下，在无钙条件下也以浓度依赖的方式诱导 MMPT 孔开放，分别为 5%、3.7%、-42.6%、-81.5%、-187%和-161.1%。目前的观察结果证实了强啡肽 B 和对羟基苯酚的抗氧化性能，并表明这些化合物在治疗需要诱导细胞死亡的疾病（包括癌症）方面具有潜在的治疗用途。
