Gerasimova Yulia V, Ballantyne Jack, Kolpashchikov Dmitry M
Department of Chemistry, University of Central Florida, Orlando, FL, USA.
Methods Mol Biol. 2013;1039:69-80. doi: 10.1007/978-1-62703-535-4_5.
Hybridization-based techniques have been extensively employed for the analysis of specific DNA/RNA sequences. Herein, we describe highly specific inexpensive smart hybridization-based sensor that takes advantage of a universal molecular beacon probe as a fluorescent reporter. The sensor has a straightforward design, and demonstrates improved selectivity and specificity of nucleic acid recognition. It is cost-efficient since it utilizes the same molecular beacon probe for the analysis of many nucleic acid sequences.
基于杂交的技术已被广泛用于特定DNA/RNA序列的分析。在此，我们描述了一种高度特异且廉价的基于智能杂交的传感器，它利用通用分子信标探针作为荧光报告分子。该传感器设计简单，在核酸识别方面表现出更高的选择性和特异性。由于它使用相同的分子信标探针来分析多种核酸序列，因此具有成本效益。
