Smolina Irina
Department of Biomedical Engineering, Boston University, Boston, MA, USA.
Methods Mol Biol. 2013;1039:223-31. doi: 10.1007/978-1-62703-535-4_18.
Peptide nucleic acid (PNA) openers have the unique ability to invade double-stranded DNA with high efficiency and sequence specificity, making it possible to detect short (about 20 bp), single-copy bacterial DNA sequences. PNA openers bind to a target signature site on one strand of bacterial DNA, leaving the other strand open for hybridization with a circularizable oligonucleotide probe. The assembled complex serves as a template for rolling circle amplification. The obtained amplicon is decorated with short, single-stranded DNA probes carrying fluorophores and detected via fluorescence microscopy.
肽核酸（PNA）开启子具有独特能力，能够高效且序列特异性地侵入双链DNA，从而有可能检测短的（约20个碱基对）单拷贝细菌DNA序列。PNA开启子与细菌DNA一条链上的目标特征位点结合，使另一条链保持开放以便与可环化的寡核苷酸探针杂交。组装好的复合物作为滚环扩增的模板。所获得的扩增子用携带荧光团的短单链DNA探针进行标记，并通过荧光显微镜检测。
