笼形荧光核苷酸在活细胞RNA成像中的应用。
Application of caged fluorescent nucleotides to live-cell RNA imaging.
作者信息
Okamoto Akimitsu
机构信息
The Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2013;1039:303-18. doi: 10.1007/978-1-62703-535-4_24.
A caged fluorescent nucleic acid probe, which contains a nucleotide modified with one photolabile nitrobenzyl unit and two hybridization-sensitive thiazole orange units, has been synthesized for area-specific fluorescence imaging of RNA in a cell. The probe emits very weak fluorescence regardless of the presence of the complementary RNA, whereas it shows hybridization-sensitive fluorescence emission after photoirradiation for uncaging. Such probes are generated via several chemical synthetic steps and are applicable to area-specific RNA imaging in a cell. Only probes that exist in the defined irradiation area are activated via uncaging irradiation.
一种笼状荧光核酸探针已被合成，用于细胞内RNA的区域特异性荧光成像。该探针含有一个用一个光不稳定硝基苄基单元修饰的核苷酸和两个对杂交敏感的噻唑橙单元。无论互补RNA是否存在，该探针发出的荧光都非常微弱，而在光照射以解除笼蔽后，它会显示出对杂交敏感的荧光发射。此类探针通过几个化学合成步骤生成，适用于细胞内区域特异性RNA成像。只有存在于定义的照射区域内的探针通过解除笼蔽照射被激活。
Zotero 插件