Liu Li, Tang Bing, Huang Shao-Song, Fu Feng-Lian, Zhang Qi-Qin, Li Jian-Bin, Luo Jian-Zhong
School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China.
Huan Jing Ke Xue. 2013 Jul;34(7):2869-75.
The present work focused on a rapid enrichment and cultivation of denitrifying phosphate-removal bacteria (DPB) in a membrane bio-reactor(MBR) by using A2/O anaerobic sludge from a wastewater treatment plant as seed, as well as providing an identification method. In the experiments, sodium acetate was used as the carbon source and a certain amount of nitrate was added to the MBR in the anoxic stage. Results showed that, with the efficient trap of the hollow-fiber membrane module, the proportion of DPB in all the phosphate-accumulating organisms (PAOs) increased from 24% to 93% within 35 days after two-stage's cultivation including anaerobic/aerobic and anaerobic/anoxic, during which the removal efficiency of nitrogen and phosphorus reached more than 90%. The activated sludge was identified by combining a regular method and the fluorescence in situ hybridization (FISH) technique, which demonstrated that Pseudomonas sp. and Rhodocyclus sp. were the dominant bacteria in the used bioreactor.
本研究以某污水处理厂的A2/O厌氧污泥为种子，致力于在膜生物反应器（MBR）中快速富集和培养反硝化聚磷菌（DPB），并提供一种鉴定方法。实验中，以乙酸钠为碳源，在缺氧阶段向MBR中添加一定量的硝酸盐。结果表明，通过中空纤维膜组件的高效截留，经过厌氧/好氧和厌氧/缺氧两阶段培养，在35天内，所有聚磷菌（PAO）中DPB的比例从24%增加到93%，在此期间氮和磷的去除效率达到90%以上。通过常规方法与荧光原位杂交（FISH）技术相结合对活性污泥进行鉴定，结果表明假单胞菌属和红环菌属是所用生物反应器中的优势菌。
