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[扑草净降解菌的分离、筛选、鉴定及其降解特性]

[Isolation, screening and identification of prometryne-degrading bacteria and their degrading characteristics].

作者信息

Zhou Ji-Hai, Sun Xiang-Wu, Hu Feng, Li Hui-Xin

机构信息

School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China.

出版信息

Huan Jing Ke Xue. 2013 Jul;34(7):2894-8.

Abstract

The purpose of this study was to develop some bacterial strains that could degrade prometryne effectively. Bacteria were screened and purified from sediments of a pesticide plant with the method of domestication with gradient concentrations. Two strains were isolated and named P-1 and P-2, respectively. According to their morphology, physiological, biochemical properties and the analysis of their 16S rRNA gene sequences, the strain P-1 is Gram negative and identified as Ochrobactrum; the strain P-2 is Gram positive and identified as Bacillus. GenBank Accession numbers are HM004554 and HM004555, respectively. Degradation rates of the strains P-1 and P-2 for prometryne concentration with 40 mg x L(-1) were 46.5% and 65.4% after 12 days in the liquid culture experiment, respectively. This study provides an essential material and theoretical foundation for the remediation of prometryne contaminated soils.

摘要

本研究的目的是培育出一些能够有效降解扑草净的菌株。采用梯度浓度驯化法从一家农药厂的沉积物中筛选并纯化细菌。分离出两株菌株,分别命名为P-1和P-2。根据它们的形态、生理生化特性以及16S rRNA基因序列分析,菌株P-1为革兰氏阴性菌,鉴定为苍白杆菌属;菌株P-2为革兰氏阳性菌,鉴定为芽孢杆菌属。GenBank登录号分别为HM004554和HM004555。在液体培养实验中,12天后菌株P-1和P-2对浓度为40 mg·L⁻¹的扑草净的降解率分别为46.5%和65.4%。本研究为扑草净污染土壤的修复提供了重要的材料和理论基础。

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