Gao Sha, Jin De-Cai, Zhao Zhi-Rui, Qi Rong, Peng Xia-Wei, Bai Zhi-Hui
College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Huan Jing Ke Xue. 2013 Jul;34(7):2912-7.
The anoxic-anaerobic-oxic (A2O) process is widely used in wastewater treatment plant, however, sludge bulking and foaming are the most frequent operational problems in this process. Activated sludge bulking is caused by the overgrowth of some types of filamentous bacteria, especially Microthrix parvicella. In the study, 17 strains of filamentous bacteria were isolated from the bulking sludge of A2O process using Gause's medium. The 16S rRNA genes of the 17 isolates were sequenced to analyze their diversity. The results showed all of the 17 isolates were Streptomyces. Further analysis of these strains by the repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that there was a high diversity in these isolated Streptomyces. The physiological properties of them were different from Microthrix parvicella. The settleability of activated sludge was improved when some of the isolates were inoculated.
缺氧-厌氧-好氧（A2O）工艺在污水处理厂中被广泛应用，然而，污泥膨胀和泡沫是该工艺中最常见的运行问题。活性污泥膨胀是由某些丝状细菌过度生长引起的，尤其是微小丝菌。在本研究中，使用高斯培养基从A2O工艺的膨胀污泥中分离出17株丝状细菌。对这17株分离菌的16S rRNA基因进行测序以分析其多样性。结果表明，这17株分离菌均为链霉菌。基于聚合酶链反应（rep-PCR）技术对这些菌株进行的重复序列进一步分析表明，这些分离出的链霉菌具有高度多样性。它们的生理特性与微小丝菌不同。接种部分分离菌后，活性污泥的沉降性能得到改善。
