State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
J Anim Sci Biotechnol. 2013 Sep 12;4(1):34. doi: 10.1186/2049-1891-4-34.
This study examined the effects of various levels of dietary nonphytate phosphorus on laying performance and the expression patterns of phosphorus metabolism related genes in Dwarf pink-shell laying hens. A total of 405 28-week-old Dwarf pink-shell laying hens were fed the same corn-soybean basal meals but containing 0.20%, 0.25%, 0.30%, 0.35% or 0.40% nonphytate phosphorus. The results showed that feed intake, egg production, and average egg weights were quadratically correlated with dietary nonphytate phosphorus content (P < 0.05), and the highest egg production, feed intake and average egg weights were achieved when dietary nonphytate phosphorus was at 0.3% (P < 0.05). mRNA expression of intestinal sodium phosphorus co-transporter linearly decreased when dietary nonphytate phosphorus increased. mRNA and protein expression of intestinal calbindin and vitamin D receptor correlated quadratically with dietary nonphytate phosphorus, and the highest expression was found when dietary available phosphorus was at 0.25% to 0.3%. In conclusion, the ideal phosphorus requirement for Dwarf pink-shell layer hens is estimated to be 0.3% in a corn-soybean diet. With this level of phosphorus supplementation, calbindin and vitamin D receptor reached their highest expression.
本研究旨在探讨不同水平的非植酸磷对矮粉壳蛋鸡产蛋性能和磷代谢相关基因表达模式的影响。选用 405 只 28 周龄矮粉壳蛋鸡，饲喂相同的玉米-豆粕基础日粮，但分别含有 0.20%、0.25%、0.30%、0.35%或 0.40%的非植酸磷。结果表明，饲粮非植酸磷含量与采食量、产蛋率和平均蛋重呈二次曲线相关（P < 0.05），当饲粮非植酸磷含量为 0.3%时产蛋率、采食量和平均蛋重最高（P < 0.05）。随着饲粮非植酸磷的增加，肠道钠磷共转运体的 mRNA 表达呈线性下降。肠道钙结合蛋白和维生素 D 受体的 mRNA 和蛋白表达与饲粮非植酸磷呈二次相关，当饲粮有效磷为 0.25%~0.3%时表达最高。综上所述，矮粉壳蛋鸡的理想磷需要量估计在玉米-豆粕日粮中为 0.3%，在此磷添加水平下，钙结合蛋白和维生素 D 受体表达最高。
