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在含琼脂糖凝胶的抗血清中用电免疫扩散法定量测定房水蛋白质(作者译)

[Quantitative determination of aqueous humor proteins by electroimmodiffusion in an antiserum containing agarosegel (author's transl)].

作者信息

Zirm M, Petek W, Schmut O, Hofmann H, Holasek A

出版信息

Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1975 Aug 6;196(2):127-32. doi: 10.1007/BF00414798.

Abstract

An exact quantitation of aqueous humor proteins within a few hours can be attained by an modification of the quantitative estimation of the proteins by electrophoresis in agarose gel established by Laurell. The aqueous humor proteins form long immunoprecipitations in agargel. The length of the precipitats is proportional to the concentration. By determination of albumin, coeruloplasmin and transferrin the application can be pointed out.

摘要

通过对劳雷尔建立的琼脂糖凝胶电泳蛋白质定量估计方法进行改进,可在数小时内实现房水蛋白质的精确定量。房水蛋白质在琼脂凝胶中形成长免疫沉淀。沉淀的长度与浓度成正比。通过测定白蛋白、铜蓝蛋白和转铁蛋白可指出该应用。

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