Roy Sashwati, Sen Chandan K
Department of Surgery, Comprehensive Wound Center, The Ohio State University, Columbus, OH, USA.
Methods Mol Biol. 2013;1037:233-43. doi: 10.1007/978-1-62703-505-7_12.
Direct procurement of tissue samples from clinically presented chronic human wounds is a powerful approach to understand mechanism at play in an actual problem wound. While such approach suffers from limitations related to lack of reproducible conditions across wounds, something that we are used to in the laboratory while studying wounds on experimental animals, the direct study of human wound tissue helps recognize the right questions to ask in the laboratory. Going back and forth between human wound and experimental animal studies helps steer studies on experimental wounds in a clinically relevant direction. In this chapter, we describe critical factors that need to be considered prior to planning a study involving human wound samples. In addition, we describe an approach to capture wound hyperproliferative epithelium (HE) from chronic human wound biopsies using laser capture microdissection (LCM). LCM is a new technology applicable to a broad range of clinical research and represents a catalyst of sophisticated translational research.
从临床上出现的慢性人类伤口直接获取组织样本，是了解实际问题伤口中所起机制的有力方法。虽然这种方法存在与不同伤口间缺乏可重复条件相关的局限性，而这是我们在实验室研究实验动物伤口时所习惯的，但对人类伤口组织的直接研究有助于确定在实验室中要提出的正确问题。在人类伤口研究和实验动物研究之间反复进行，有助于将实验伤口的研究导向临床相关方向。在本章中，我们描述了在计划涉及人类伤口样本的研究之前需要考虑的关键因素。此外，我们描述了一种使用激光捕获显微切割（LCM）从慢性人类伤口活检中获取伤口过度增殖上皮（HE）的方法。LCM是一种适用于广泛临床研究的新技术，是精密转化研究的催化剂。
