Key Lab of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, No. 29, Wangjiang Road, Chengdu, Sichuan 610064, China.
Analyst. 2013 Nov 7;138(21):6558-64. doi: 10.1039/c3an01343j.
In this article, we established a fluorescent chiral recognition and detection method for cysteine based on the L-glutathione (L-GSH)-stabilized Ag nanoclusters (AgNCs) with high selectivity and sensitivity. A fast and green microwave (MW)-assisted strategy has been employed for synthesizing water-soluble fluorescent L-GSH-AgNCs. The reaction time was shortened from hours to several minutes. The synthetic process utilized L-glutathione as a stabilizing agent and a reducing agent without any other toxic reducing agent, such as NaBH4 or N2H4. The method is environmental friendly. Due to the different responses to AgNCs from chiral cysteine, we found d-cysteine hardly affects the fluorescence intensity of the AgNCs, whereas L-cysteine distinctly weakened its fluorescence intensity. This experiment indicated that fluorescence quenching efficiency of AgNCs was proportional to the concentration of L-cysteine in the range from 0.025 to 50 μmol L(-1).
在本文中，我们建立了一种基于 L-谷胱甘肽（L-GSH）稳定的银纳米簇（AgNCs）的对半胱氨酸具有高选择性和高灵敏度的荧光手性识别和检测方法。采用快速、绿色的微波（MW）辅助策略合成了水溶性荧光 L-GSH-AgNCs。反应时间从数小时缩短到几分钟。该合成过程以 L-谷胱甘肽作为稳定剂和还原剂，而不使用任何其他有毒还原剂，如 NaBH4 或 N2H4。该方法环保。由于手性半胱氨酸对 AgNCs 的不同响应，我们发现 d-半胱氨酸几乎不影响 AgNCs 的荧光强度，而 L-半胱氨酸则明显减弱了其荧光强度。该实验表明，AgNCs 的荧光猝灭效率与 L-半胱氨酸的浓度成正比，范围从 0.025 到 50 μmol L(-1)。
