Key Laboratory of Leather Chemistry and Engineering, Education Ministry, College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
World J Microbiol Biotechnol. 2014 Feb;30(2):605-12. doi: 10.1007/s11274-013-1472-4. Epub 2013 Sep 13.
The aim of this study was to investigate and compare the microbial community structures of eubacteria and archaea in the pit mud of Chinese Luzhou-flavor liquor from the wall (C(w)) and bottom (C(b)) of cellar through nested PCR-denaturing gradient gel electrophoresis (DGGE). The Shannon-Wiener index (H) calculated from the DGGE profiles showed that the community diversities of eubacteria and archaea in samples from C(b) were almost higher than that from C(w). In addition, cluster analysis of the DGGE profiles revealed that some differences were found in the microbial community structure in samples from different locations. The closely relative microorganisms of all eubacterial 16S rRNA gene sequences fell into four phyla (Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria), including 12 genera and 2 uncultured eubacteria. Moreover, 37.1% eubacteria were affiliated with Clostridium. Particularly, genus Acinetobacter was absent in all samples from C(b) but present in all samples from C(w). The closely relative microorganisms of all archaeal 16S rRNA gene sequences fell into four genera, which included Methanobrevibacter, Methanoculleus, Methanobacterium and Methanosaeta, while the dominant archaea in samples from C(w) and C(b) were similar. Results presented in this study provide further understanding of the spatial differences in microbial community structure in the pit mud, and is of great importance for the production and quality improvement of Luzhou-flavor liquor.
本研究旨在通过巢式 PCR-变性梯度凝胶电泳 (DGGE) 技术，调查和比较中国浓香型白酒窖池壁（C(w)）和池底（C(b)）窖泥中真细菌和古菌的微生物群落结构。从 DGGE 图谱计算得出的 Shannon-Wiener 指数 (H) 表明，C(b) 样品中真细菌和古菌的群落多样性几乎均高于 C(w) 样品。此外，DGGE 图谱的聚类分析表明，不同位置样品的微生物群落结构存在一些差异。所有细菌 16S rRNA 基因序列的密切相关微生物属于 4 个门（Firmicutes、Proteobacteria、Bacteroidetes 和 Actinobacteria），包括 12 个属和 2 个未培养细菌。此外，37.1%的细菌与梭菌属有关。特别是，所有 C(b) 样品中均不存在不动杆菌属，但所有 C(w) 样品中均存在不动杆菌属。所有古菌 16S rRNA 基因序列的密切相关微生物属于四个属，包括 Methanobrevibacter、Methanoculleus、Methanobacterium 和 Methanosaeta，而 C(w) 和 C(b) 样品中的优势古菌相似。本研究结果进一步了解了窖泥中微生物群落结构的空间差异，对浓香型白酒的生产和质量提高具有重要意义。
