Trautwein E A, Hayes K C
Foster Biomedical Research Laboratory, Brandeis University, Waltham, MA 02254.
Am J Clin Nutr. 1990 Oct;52(4):758-64. doi: 10.1093/ajcn/52.4.758.
The "normal" physiological taurine status in human plasma and whole blood was evaluated for day-to-day variation. Because plasma taurine concentrations can vary by greater than or equal to 100% because of sampling and analytical techniques, various blood-collecting and -handling procedures were compared to ascertain the most reliable method for measuring plasma taurine. Blood collection into EDTA-wetted plastic syringes at room temperature proved most reliable. Plasma and whole-blood taurine concentrations were determined in the same 13 women and 11 men three times within a 10-d period. The normal plasma taurine concentration was 44 +/- 9 mumol/L (mean +/- SD; n = 40) in fasting subjects and 42 +/- 7 mumol/L (n = 30) in semi-fasted individuals, with an intraindividual variation of 9 +/- 5%. Whole-blood taurine concentrations ranged from 164 to 318 mumol/L and had an intraindividual variation of 11 +/- 5%. Inter-individual variation averaged 12 +/- 7% for plasma and 7 +/- 6% for whole blood taurine. Because plasma and whole-blood taurine concentrations were not correlated, assessment of both would provide the most accurate estimate of taurine status. Short of that, whole-blood taurine would appear to be the best single measure.
评估了人血浆和全血中“正常”生理牛磺酸状态的每日变化情况。由于采样和分析技术的原因，血浆牛磺酸浓度的变化可能大于或等于100%，因此对各种采血和处理程序进行了比较，以确定测量血浆牛磺酸最可靠的方法。结果表明，在室温下将血液采集到用乙二胺四乙酸（EDTA）湿润的塑料注射器中最为可靠。在10天内，对13名女性和11名男性进行了三次相同的检测，测定其血浆和全血中的牛磺酸浓度。空腹受试者的正常血浆牛磺酸浓度为44±9μmol/L（平均值±标准差；n = 40），半空腹个体为42±7μmol/L（n = 30），个体内变异为9±5%。全血牛磺酸浓度范围为164至318μmol/L，个体内变异为11±5%。血浆牛磺酸的个体间变异平均为12±7%，全血牛磺酸为7±6%。由于血浆和全血中的牛磺酸浓度不相关，因此同时评估两者将能最准确地估计牛磺酸状态。若无法同时评估，全血牛磺酸似乎是最佳的单一测量指标。
