Department of Microbiology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research , Balawala, Dehradun, Uttarakhand , India, 248161.
Braz J Microbiol. 2012 Jan;43(1):205-14. doi: 10.1590/S1517-838220120001000022. Epub 2012 Jun 1.
Actinomycetes from earthworm castings were isolated and screened for their antimicrobial activity and industrial enzymes. A total of 48 isolates were obtained from 12 samples of earthworm castings. Highest numbers of isolates were recovered from forest site (58.33 %) as compared to grassland (25%) and agricultural land (16.66%). The growth patterns, mycelial coloration of abundance actinomycetes were documented. The dominant genera Identified by cultural, morphological and physiological characteristics were Streptomyces (60.41%) followed by Streptosporangium (10.41%),Saccharopolyspora (6.25%) and Nocardia (6.25%). Besides these, other genera like Micromonospora, Actinomadura, Microbispora, Planobispora and Nocardiopsis were also recovered but in low frequency. Among the 48 isolates, 52.08% were found active against one or more test organisms. Out of 25 active isolates 16% showed activity against bacterial, human fungal as well as phytopathogens. Among 48 isolates 38, 32, 21, 20, 16 and 14 produced enzyme amylase, caseinase, cellulase, gelatinase, xylanase and lipase respectively while 10 isolates produced all the enzymes. More interestingly 2, 3, and 1 isolates produced amylase, xylanase and lipase at 45°C respectively. In the view of its antimicrobial activity as well as enzyme production capability the genus Streptomyces was dominant. The isolate EWC 7(2) was most promising on the basis of its interesting antimicrobial activity and was identified as Streptomyces rochei. The results of these findings have increased the scope of finding industrially important actinomycetes from earthworm castings and these organisms could be promising sources for industrially important molecules or enzymes.
从蚯蚓粪中分离筛选出具有抗菌活性和工业酶活性的放线菌。从 12 个蚯蚓粪样本中获得了总共 48 个分离株。与草原（25%）和农田（16.66%）相比，从森林地区（58.33%）获得的分离株数量最多。记录了丰富放线菌的生长模式和菌丝颜色。通过文化、形态和生理特征鉴定出的主要属为链霉菌（60.41%），其次为链孢囊菌（10.41%）、糖多孢菌（6.25%）和诺卡氏菌（6.25%）。除此之外，还回收了其他属，如微单胞菌、放线菌、小单胞菌、拟无枝酸菌和诺卡氏菌，但频率较低。在 48 个分离株中，有 52.08%的分离株对一种或多种测试生物具有活性。在 25 个活性分离株中，有 16%对细菌、人类真菌和植物病原菌表现出活性。在 48 个分离株中，有 38、32、21、20、16 和 14 个分离株分别产生了淀粉酶、酪蛋白酶、纤维素酶、明胶酶、木聚糖酶和脂肪酶，而 10 个分离株产生了所有这些酶。更有趣的是，有 2、3 和 1 个分离株分别在 45°C 下产生了淀粉酶、木聚糖酶和脂肪酶。就其抗菌活性和产酶能力而言，链霉菌属占主导地位。根据其有趣的抗菌活性，分离株 EWC 7(2) 被认为是罗氏链霉菌。这些发现的结果增加了从蚯蚓粪中寻找具有工业重要性的放线菌的范围，这些生物可能是工业重要分子或酶的有前途的来源。
