Ahn C, Stiles M E
Department of Food Science, University of Alberta, Edmonton, Canada.
Appl Environ Microbiol. 1990 Aug;56(8):2503-10. doi: 10.1128/aem.56.8.2503-2510.1990.
Carnobacterium piscicola LV17 isolated from vacuum-packed meat produces bacteriocin(s) that is active against closely related lactic acid bacteria, Enterococcus spp., and a strain of Listeria monocytogenes but not against gram-negative bacteria. The bacteriocin has a bactericidal mode of action, is heat resistant, and is stable over a wide range of pH but is inactivated by proteolytic enzymes. Sensitive and resistant cells were shown to adsorb the bacteriocin, but cell death depended on contact of the bacteriocin with the cell membrane. Bacteriocin production is detected early in the growth cycle of the organism in APT broth, but it is not produced in APT broth adjusted to pH 5.5. Bacteriocin production and resistance to the bacteriocin produced are associated with two plasmids of 40 and 49 megadaltons. The possibility that two bacteriocins are produced is indicated because the inhibitory substances of the mutant strains containing either the 40- or 49-megadalton plasmids have different antimicrobial spectra.
从真空包装肉中分离出的嗜鱼肉杆菌LV17可产生对密切相关的乳酸菌、肠球菌属以及一株单核细胞增生李斯特菌有活性，但对革兰氏阴性菌无活性的细菌素。该细菌素具有杀菌作用方式，耐热，在很宽的pH范围内稳定，但会被蛋白水解酶灭活。敏感细胞和抗性细胞均能吸附该细菌素，但细胞死亡取决于细菌素与细胞膜的接触。在APT肉汤中，细菌素的产生在该生物体生长周期早期即可检测到，但在pH值调至5.5的APT肉汤中不产生。细菌素的产生以及对所产生细菌素的抗性与两个大小分别为40和49兆道尔顿的质粒相关。由于含有40兆道尔顿或49兆道尔顿质粒的突变菌株的抑制物质具有不同的抗菌谱，因此表明可能产生了两种细菌素。
