Department of Respiratory Medicine, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210001, China.
Chin Med J (Engl). 2013;126(17):3259-63.
Genioglossal dysfuntion takes an important role in pathogenesis of obstructive sleep apnea hypopnea syndrome (OSAHS) in which chronic intermittent hypoxia (CIH) is the major pathological origin. Recent studies have suggested genioglossal injury induced by CIH might be improved by adiponectin. The aim of this study was to investigate the effects of adiponectin on genioglossus contractile properties in rats exposed to CIH.
Thirty-nine healthy male Wistar rats were randomly divided into three groups: normal control (NC), CIH and adiponectin supplement (CIH+Ad) with 13 rats in each. Rats in NC were kept breathing normal air, while rats in CIH and CIH+Ad experienced the same CIH environment eight hours per day for 35 successive days. Rats in CIH+Ad were given intravenous adiponectin of 10 µg twice a week for 30 successive days. Rats in the NC and CIH were injected with normal saline as a control. After 35 days' CIH exposure, the levels of serum adiponectin and genioglossus contractile properties were compared.
Serum adiponectin level was significantly lower in CIH than in NC (1210 ng/ml vs. 2236 ng/ml). Serum adiponectin level in CIH+Ad (1844 ng/ml) was significantly higher than CIH but lower than NC. Twitch tension, time to peak tension, half relaxation time and tetanic tension were significantly lower in CIH than NC and improved in CIH+Ad. All mean tetanic fatigue indices decreased more rapidly in the first 20 seconds than during the subsequent 100 seconds. Tetanic fatigue indices in NC and CIH+Ad were significantly higher compared to CIH.
CIH could lead to hypoadiponectinaemia, impaired genioglossus contractile properties and decreased fatigue resistance in rats. Such changes could be partially offset by supplementation of adiponectin.
颏舌肌功能障碍在阻塞性睡眠呼吸暂停低通气综合征（OSAHS）的发病机制中起着重要作用，其中慢性间歇性低氧（CIH）是主要的病理起源。最近的研究表明，CIH 引起的颏舌肌损伤可能会被脂联素改善。本研究旨在探讨脂联素对 CIH 大鼠颏舌肌收缩性能的影响。
39 只健康雄性 Wistar 大鼠随机分为三组：正常对照组（NC）、CIH 组和脂联素补充组（CIH+Ad），每组 13 只。NC 组大鼠呼吸正常空气，CIH 组和 CIH+Ad 组大鼠每天接受相同的 CIH 环境 8 小时，连续 35 天。CIH+Ad 组大鼠每周两次静脉注射脂联素 10μg，连续 30 天。NC 和 CIH 组大鼠注射生理盐水作为对照。35 天后，比较血清脂联素水平和颏舌肌收缩性能。
与 NC 组相比，CIH 组血清脂联素水平显著降低（1210ng/ml 比 2236ng/ml）。CIH+Ad 组（1844ng/ml）血清脂联素水平高于 CIH 组，但低于 NC 组。与 NC 组相比，CIH 组的肌颤搐张力、达峰时间、半松弛时间和强直张力均显著降低，CIH+Ad 组则有所改善。所有平均强直疲劳指数在前 20 秒下降速度均快于随后的 100 秒。NC 组和 CIH+Ad 组的强直疲劳指数均显著高于 CIH 组。
CIH 可导致大鼠脂联素血症降低、颏舌肌收缩功能障碍和疲劳抵抗力下降。脂联素的补充可部分抵消这些变化。
