Bryant D A, Stirewalt V L
Dept. of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
FEBS Lett. 1990 Jan 1;259(2):273-80. doi: 10.1016/0014-5793(90)80026-f.
The rpl35, rpl20, rpl5, rps8, and a portion of the rpl6 genes of the cyanelle genome of Cyanophora paradoxa have been cloned, mapped and sequenced. Homologs of the rpl35, rpl5, and rpl6 genes are not found in the chloroplasts of higher plants. The rpl35 genes most likely form a dicistronic operon which is located upstream from the apcE-apcA-apcB locus of the cyanelle and which is divergently transcribed from this locus. The rpl5, rpl8, and rpl6 genes probably form a part of a larger cluster of genes encoding components of the cyanellar ribosomes. These genes are organized in a fashion similar to that observed in all procaryotes examined to date, with the exception that the rps14 gene is not found between the rpl5 and rps8 coding sequences. Hypotheses concerning the origins of cyanelles and chloroplasts are discussed.
已经对蓝氏原绿藻蓝小体基因组的rpl35、rpl20、rpl5、rps8以及部分rpl6基因进行了克隆、定位和测序。在高等植物的叶绿体中未发现rpl35、rpl5和rpl6基因的同源物。rpl35基因很可能形成一个双顺反子操纵子，该操纵子位于蓝小体的apcE - apcA - apcB基因座上游，并从该基因座反向转录。rpl5、rpl8和rpl6基因可能构成编码蓝小体核糖体组分的更大基因簇的一部分。这些基因的组织方式与迄今所研究的所有原核生物中观察到的方式相似，不同之处在于在rpl5和rps8编码序列之间未发现rps14基因。讨论了关于蓝小体和叶绿体起源的假说。
