Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, PR China.
Biosens Bioelectron. 2014 Feb 15;52:438-44. doi: 10.1016/j.bios.2013.08.023. Epub 2013 Aug 24.
Carbon nanomaterials are typically used in electrochemical biosensing applications for their unique properties. We report a hollow graphitic nanocapsule (HGN) utilized as an efficient electrode material for sensitive hydrogen peroxide detection. Methylene blue (MB) molecules could be efficiently adsorbed on the HGN surfaces, and this adsorption capability remained very stable under different pH regimes. HGNs were used as three-dimensional matrices for coimmobilization of MB electron mediators and horseradish peroxidase (HRP) to build an HGN-HRP-MB reagentless amperometric sensing platform to detect hydrogen peroxide. This simple HGN-HRP-MB complex demonstrated very sensitive and selective hydrogen peroxide detection capability, as well as high reproducibility and stability. The HGNs could also be utilized as matrices for immobilization of other enzymes, proteins or small molecules and for different biomedical applications.
碳纳米材料因其独特的性质，通常被用于电化学生物传感应用中。我们报道了一种空心石墨纳米胶囊（HGN），它可用作高效的电极材料，用于灵敏的过氧化氢检测。亚甲基蓝（MB）分子可以有效地被吸附在 HGN 表面上，并且在不同的 pH 条件下，这种吸附能力仍然非常稳定。HGN 可用作共固定化 MB 电子介体和辣根过氧化物酶（HRP）的三维基质，构建无试剂的 HGN-HRP-MB 安培传感平台，以检测过氧化氢。这种简单的 HGN-HRP-MB 复合物表现出非常灵敏和选择性的过氧化氢检测能力，以及高重现性和稳定性。HGN 还可用作固定其他酶、蛋白质或小分子的基质，用于不同的生物医学应用。
