Yasmin Firdous, Akhtar Naeem, Hameed Abdul
Microbiology Laboratory, Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
Pak J Pharm Sci. 2013 Sep;26(5):1041-4.
Pseudomonas aeruginosa is an increasingly prevalent nosocomial human pathogen. Infections with multidrug-resistant (MDR) P. aeruginosa are currently a treatment challenge and requires search for better treatment options.
To determine in vitro synergistic effect of ciprofloxacin in combination with amikacin and gentamicin against MDR P. aeruginosa clinical isolates.
Antibiotic resistance pattern of 100 identified clinical isolates of P. aeruginosa was determined against eight antibiotics by disc diffusion method at Microbiology Laboratory, Holy Family Hospital, Rawalpindi. For 30 selected MDR isolates, minimum inhibitory concentrations (MICs) of amikacin and gentamicin were determined separately by agar diffusion method followed by combined activity of ciprofloxacin with amikacin and gentamicin by checkerboard agar dilution technique.
Antibiotic resistance pattern of P. aeruginosa isolates was; gentamicin and carbenicillin (94%), amikacin and piperacillin (92%), ceftazidime (90%), colistin (87%), ciprofloxacin (79%) and imipenem (72%). MICs against 30 selected MDR isolates ranged from 32 to >128μg/mL for amikacin, and >128μg/mL for gentamicin. Synergistic effect was observed in 12/30(40%) isolates for AK+CIP and in 05/30 (16.7%) for CN+CIP.
Ciprofloxacin in combination with amikacin and gentamicin showed synergistic effect and no antagonistic effect against MDR P. aeruginosa.
铜绿假单胞菌是一种在医院中日益普遍的人类病原体。耐多药铜绿假单胞菌感染目前是一个治疗挑战，需要寻找更好的治疗选择。
确定环丙沙星与阿米卡星和庆大霉素联合对耐多药铜绿假单胞菌临床分离株的体外协同作用。
在拉瓦尔品第圣家医院微生物实验室，采用纸片扩散法测定了100株已鉴定的铜绿假单胞菌临床分离株对8种抗生素的耐药模式。对于30株选定的耐多药分离株，通过琼脂扩散法分别测定阿米卡星和庆大霉素的最低抑菌浓度（MIC），然后采用棋盘琼脂稀释技术测定环丙沙星与阿米卡星和庆大霉素的联合活性。
铜绿假单胞菌分离株的耐药模式为：庆大霉素和羧苄西林（94%）、阿米卡星和哌拉西林（92%）、头孢他啶（90%）、黏菌素（87%）、环丙沙星（79%）和亚胺培南（72%）。对30株选定的耐多药分离株，阿米卡星的MIC范围为32至>128μg/mL，庆大霉素的MIC范围为>128μg/mL。在12/30（40%）的分离株中观察到AK+CIP有协同作用，在05/30（16.7%）的分离株中观察到CN+CIP有协同作用。
环丙沙星与阿米卡星和庆大霉素联合对耐多药铜绿假单胞菌显示出协同作用，无拮抗作用。
