Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, ROC.
Anal Biochem. 2013 Dec 15;443(2):169-71. doi: 10.1016/j.ab.2013.09.004. Epub 2013 Sep 11.
The presented approach provides the advanced development of effective, rapid, and versatile electrochemical sensors for a small amount of analytes on potential, cheap, and disposable printed chips. The electrocatalytic activity of this biosensor revealed the feasible detection of hydrogen peroxide at low potential (~0.09 V) and the detection of a biocontaminant inhibitor (organophosphorus pesticide) in a wide range of concentrations. This efficiency comes from the chemical immobilization of catalysts (Pt nanoparticles) and electron transfer-enlarging materials (carbon nanotubes) on an electrode. Especially, dendrimers raise the stable conjugation of enzymes (acetylcholinesterase/choline oxidase/peroxidase) as well as nanoparticles and carbon nanotubes on an electrode.
所提出的方法为在电势、廉价和一次性印刷芯片上对小量分析物进行有效、快速和多功能电化学传感器提供了先进的发展。该生物传感器的电催化活性显示出在低电势（~0.09 V）下对过氧化氢的可行检测以及在宽浓度范围内对生物污染物抑制剂（有机磷农药）的检测。这种效率来自于催化剂（铂纳米粒子）和电子转移增大材料（碳纳米管）在电极上的化学固定化。特别是，树枝状大分子提高了酶（乙酰胆碱酯酶/胆碱氧化酶/过氧化物酶）以及纳米粒子和碳纳米管在电极上的稳定结合。
