Department of Biosciences and Health Sciences, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310, Johor Bahru, Johor, Malaysia.
Appl Biochem Biotechnol. 2013 Dec;171(8):2247-61. doi: 10.1007/s12010-013-0490-x. Epub 2013 Sep 15.
The ability of non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment to adsorb As (III) from aqueous solution in batch experiments was investigated as a function of contact time, initial As (III) concentration, pH, temperature and biomass dosage. Langmuir model fitted the equilibrium data better in comparison to Freundlich isotherm. The maximum biosorption capacity of the sorbent, as obtained from the Langmuir isotherm, was 153.41 mg/g. The sorption kinetic of As (III) biosorption followed well the pseudo-second-order rate equation. The Fourier transform infrared spectroscopy analysis indicated the involvement of hydroxyl, amide and amine groups in As (III) biosorption process. Field emission scanning electron microscopy-energy dispersive X-ray analysis of the non-living B. cereus SZ2 biomass demonstrated distinct cell morphological changes with significant amounts of As adsorbed onto the cells compared to non-treated cells. Desorption of 94 % As (III) was achieved at acidic pH 1 showing the capability of non-living biomass B. cereus SZ2 as potential biosorbent in removal of As (III) from arsenic-contaminated mining effluent.
从金矿环境中分离出的砷耐受芽孢杆菌 SZ2 的非活体生物质在批处理实验中具有从水溶液中吸附砷 (III) 的能力，研究了其作为接触时间、初始砷 (III) 浓度、pH 值、温度和生物质剂量的函数。与 Freundlich 等温线相比，Langmuir 模型更能拟合平衡数据。从 Langmuir 等温线获得的吸附剂的最大吸附容量为 153.41mg/g。砷 (III) 吸附的吸附动力学很好地遵循拟二级速率方程。傅里叶变换红外光谱分析表明，羟基、酰胺和胺基团参与了砷 (III) 的吸附过程。对非活体芽孢杆菌 SZ2 生物质的场发射扫描电子显微镜-能谱分析表明，与未经处理的细胞相比，细胞形态发生了明显变化，并且有大量的砷吸附在细胞上。在酸性 pH 值 1 下实现了 94%的砷 (III) 解吸，表明非活体生物质芽孢杆菌 SZ2 作为一种潜在的生物吸附剂，具有从含砷矿冶废水中去除砷 (III) 的能力。
