Tomimatsu Kosuke, Shirahata Sanetaka
Faculty of Agriculture, Department of Bioscience and Biotechnology, Kyushu University, Fukuoka, Japan.
Methods Mol Biol. 2014;1060:297-307. doi: 10.1007/978-1-62703-586-6_15.
Human monoclonal antibody has great potential for treatment of various diseases utilizing their specificity against antigens. We have shown an in vitro immunization (IVI) protocol inducing antigen-specific immune responses in human peripheral blood mononuclear cells (PBMCs) for efficient production of human monoclonal antibodies. By using IVI method antigen specific antibody genes can be efficiently obtained because of increasing production of antigen-specific antibodies from in vitro immunized PBMCs. This IVI protocol will be widely applied for combination with several display methods and enhance the production of human monoclonal antibodies.
人源单克隆抗体因其对抗原的特异性而在治疗各种疾病方面具有巨大潜力。我们已经展示了一种体外免疫（IVI）方案，该方案可在人外周血单核细胞（PBMC）中诱导抗原特异性免疫反应，以高效生产人源单克隆抗体。通过使用IVI方法，由于体外免疫的PBMC产生的抗原特异性抗体增加，可以有效地获得抗原特异性抗体基因。该IVI方案将广泛应用于与多种展示方法相结合，并提高人源单克隆抗体的产量。
