Hass R, Köhler L, Rehfeldt W, Lessmann V, Müller W, Resch K, Goppelt-Struebe M
Division of Molecular Pharmacology, Medical School Hannover, Federal Republic of Germany.
Cancer Res. 1990 Jan 15;50(2):323-7.
Incubation of the human monoblastoid tumor cell line U937 with 5 x 10(-9) M 12-O-tetradecanoylphorbol-13-acetate (TPA) for 3 days was associated with marked morphological and functional changes including adherence of the cells and cessation of proliferation. While growth arrest and the viability of the cells were not influenced, the TPA-induced adherence of U937 cells could be totally blocked by incubation with tunicamycin, suggesting an involvement of N-linked carbohydrates in these cell attachment processes. The isolation and characterization of endogenous lectins with specificities for lactose, L-fucose, and D-mannose from U937 controls and TPA-differentiated U937 cells demonstrated marked differences in either the pattern and the distribution of these sugar-specific carbohydrate-binding proteins. Our results indicate that alterations in cell-surface carbohydrates are of central importance for the attachment of the differentiating U937 tumor cells.
将人单核细胞样肿瘤细胞系U937与5×10⁻⁹ M的12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）孵育3天，会伴随明显的形态和功能变化，包括细胞黏附及增殖停止。虽然细胞的生长停滞和活力未受影响，但用衣霉素孵育可完全阻断TPA诱导的U937细胞黏附，这表明N - 连接碳水化合物参与了这些细胞黏附过程。从U937对照细胞和TPA分化的U937细胞中分离并鉴定对乳糖、L - 岩藻糖和D - 甘露糖具有特异性的内源性凝集素，结果显示这些糖特异性碳水化合物结合蛋白的模式和分布存在显著差异。我们的结果表明，细胞表面碳水化合物的改变对于分化中的U937肿瘤细胞的黏附至关重要。
