Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, 60438 Frankfurt am Main (Germany).
Chembiochem. 2013 Oct 11;14(15):1991-7. doi: 10.1002/cbic.201300205. Epub 2013 Sep 3.
Six novel linear peptides, named "rhabdopeptides", have been identified in the entomopathogenic bacterium Xenorhabdus nematophila after the discovery of the corresponding rdp gene cluster by using a promoter trap strategy for the detection of insect-inducible genes. The structures of these rhabdopeptides were deduced from labeling experiments combined with detailed MS analysis. Detailed analysis of an rdp mutant revealed that these compounds participate in virulence towards insects and are produced upon bacterial infection of a suitable insect host. Furthermore, two additional rhabdopeptide derivatives produced by Xenorhabdus cabanillasii were isolated, these showed activity against insect hemocytes thereby confirming the virulence of this novel class of compounds.
在利用启动子陷阱策略检测昆虫诱导基因时，发现了昆虫病原细菌 Xenorhabdus nematophila 中的 6 种新型线性肽，命名为“rhabdopeptides”。通过标记实验和详细的 MS 分析推导出这些 rhabdopeptides 的结构。对 rdp 突变体的详细分析表明，这些化合物参与了对昆虫的毒力作用，并在合适的昆虫宿主被细菌感染时产生。此外，还从 Xenorhabdus cabanillasii 中分离出两种额外的 rhabdopeptide 衍生物，它们对昆虫血细胞具有活性，从而证实了这一新型化合物类别的毒力。
