CNRS-LAAS, 7 avenue du colonel Roche, F-31400, Toulouse, France; Université de Toulouse, UPS, LAAS, F-31400, Toulouse, France; ITAV, Centre Pierre Potier, F-31106, Toulouse, France.
Macromol Biosci. 2013 Nov;13(11):1546-55. doi: 10.1002/mabi.201300202. Epub 2013 Aug 22.
To investigate the respective roles of topography and cell/cell interactions in the development of a guided neuronal network on an engineered surface, micropatterned PDMS substrates were generated with different microgrooves geometry and investigated for the influence of cell density on neurite outgrowth and alignment. Through this systematic investigation, using a human neuronal stem cell line, the rules of neuronal network development and guiding could be learned. The results show that when cell density is increased the influence on neuritic outgrowth and alignment is very different for the various grooves geometries. The data emphasized the competition, in neurite development, between physical cues brought by surface topographical features and cell to cell communications. These results can be of particular interest for designing functional neuronal networks with a controlled architecture.
为了研究地形和细胞/细胞相互作用在引导神经原网络形成方面各自的作用，我们用不同的微沟几何图形生成了微图案化 PDMS 基底，并研究了细胞密度对轴突生长和取向的影响。通过这项系统性的研究，利用人神经干细胞系，我们可以了解神经元网络形成和引导的规律。结果表明，当细胞密度增加时，各种沟形对轴突生长和取向的影响非常不同。这些数据强调了在神经突发育过程中，表面形貌特征带来的物理线索与细胞间通讯之间的竞争。这些结果对于设计具有可控结构的功能性神经元网络可能具有特别的意义。
