Department of Pediatric Intensive Care, IRCCS Bambino Gesù Children's Hospital, Rome, Italy.
Pediatr Blood Cancer. 2014 Feb;61(2):376-9. doi: 10.1002/pbc.24753. Epub 2013 Aug 26.
We report on the successful infusion of human adenovirus (HAdV)-specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T-cell-depleted hematopoietic stem cell transplantation (HSCT) from the HLA-haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV-related respiratory failure. Donor-derived, interferon (IFN)-γ-secreting HAdV-specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T-cell transfer, viral load decreased and ECMO was discontinued. T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration.
我们报告了一例成功输注人腺病毒（HAdV）特异性 T 细胞的病例，该患者因严重 HAdV 相关呼吸衰竭在体外膜氧合（ECMO）期间接受了 HLA 单倍体相合母亲来源的 T 细胞耗竭的造血干细胞移植（HSCT）。在体外用免疫优势 HAdV5 六邻体蛋白的重叠肽刺激后，使用细胞因子捕获测定法对供体来源的 IFN-γ 分泌 HAdV 特异性 T 细胞进行了富集。T 细胞转移后两周，病毒载量下降，ECMO 停止。在四周后记录到针对 HAdV 抗原的 T 细胞反应，并与病毒清除、免疫重建和临床改善相关。
