Department of Anesthesia, General Hospital of Chengdu Military Command Area, Chengdu, Sichuan Province, P. R. China.
PLoS One. 2013 Sep 5;8(9):e73101. doi: 10.1371/journal.pone.0073101. eCollection 2013.
Liver regeneration is the basic physiological process after partial hepatectomy (PH), and is important for the functional rehabilitation of the liver after acute hepatic injury. This study was designed to explore the effects of neurolytic celiac plexus block (NCPB) on liver regeneration after PH. We established a model of PH in rats, assessing hepatic blood flow, liver function, and serum CRP, TNF-α, IL-1β and IL-6 concentrations of the residuary liver after PH. Additionally, histopathological studies, immunohistochemistry, and western blotting were also performed. Our results indicated that NCPB treatment after PH improved liver regeneration and survival rates, increased hepatic blood flow, reduced hepatocyte damage, decreased the secretion and release of inflammatory cytokines, increased the expression of B cell lymphoma/leukemia-2 (Bcl-2), and decreased the expression of Bcl-2 associated X protein (Bax). Additionally, Western blotting revealed that the expression of NF-κB p65 and c-Jun were decreased in liver after NCPB. In conclusion, the results of our present study indicate that NCPB treatment has a favorable effect on liver regeneration after PH. We suggest that NCPB can be utilized as an effective therapeutic method to help the functional rehabilitation of the liver after acute hepatic injury or liver cancer surgery.
肝再生是肝部分切除术 (PH) 后的基本生理过程，对于急性肝损伤后肝脏的功能康复非常重要。本研究旨在探讨腹腔神经丛阻断 (NCPB) 对 PH 后肝再生的影响。我们在大鼠中建立了 PH 模型，评估 PH 后残肝的肝血流量、肝功能以及血清 CRP、TNF-α、IL-1β 和 IL-6 浓度。此外，还进行了组织病理学研究、免疫组织化学和 Western blot 分析。我们的结果表明，PH 后行 NCPB 治疗可改善肝再生和存活率，增加肝血流量，减轻肝细胞损伤，减少炎症细胞因子的分泌和释放，增加 B 细胞淋巴瘤/白血病-2 (Bcl-2) 的表达，降低 Bcl-2 相关 X 蛋白 (Bax) 的表达。此外，Western blot 显示 NF-κB p65 和 c-Jun 在 NCPB 后肝组织中的表达减少。综上所述，本研究结果表明，NCPB 治疗对 PH 后肝再生具有有益的影响。我们认为，NCPB 可作为一种有效的治疗方法，有助于急性肝损伤或肝癌手术后肝脏的功能康复。
