Lafreniere R, Borkenhagen K, Bryant L D
Division of Surgical Oncology, University of Calgary, Alberta, Canada.
J Surg Oncol. 1990 Jan;43(1):8-12. doi: 10.1002/jso.2930430104.
Tumor infiltrating lymphocytes (TILs) are capable of mediating significant tumor regressions in vitro and in vivo in animal systems. In humans, however, many TIL cell lines are not cytotoxic in vitro, and clinical trials thus far have been less than encouraging. We attempted to correlate TIL cytotoxicity with time of tumor harvest and TIL cell surface antigenic expression. TILs harvested from early MC-38 adenocarcinoma tumors (days 9 and 20 post-tumor implantation), demonstrated significantly higher cytotoxicity against a variety of tumor targets compared to older TILs (days 31 and 37). The younger TILs had a higher expression of the Lyt-1 (Helper T cells), asialo GM1 (NK and T cells), and 49H.8 (NK cells) antigens. Comparison with the MCA-102 sarcoma, a tumor that does not lead to cytotoxic TILs, revealed a low expression of the Lyt-1 antigen on their cell surface. We conclude that TILs cytotoxicity is time-dependent and may be dependent on the presence of Lyt-1+ cells in the overall TIL population of cells.
肿瘤浸润淋巴细胞（TILs）在动物系统中能够在体外和体内介导显著的肿瘤消退。然而，在人类中，许多TIL细胞系在体外没有细胞毒性，并且迄今为止的临床试验效果并不理想。我们试图将TIL的细胞毒性与肿瘤收获时间以及TIL细胞表面抗原表达相关联。与较老的TIL（肿瘤植入后第31天和第37天）相比，从早期MC-38腺癌肿瘤（肿瘤植入后第9天和第20天）收获的TIL对多种肿瘤靶标的细胞毒性显著更高。较年轻的TIL中Lyt-1（辅助性T细胞）、脱唾液酸GM1（自然杀伤细胞和T细胞）和49H.8（自然杀伤细胞）抗原的表达更高。与不会产生细胞毒性TIL的MCA-102肉瘤进行比较，发现其细胞表面Lyt-1抗原的表达较低。我们得出结论，TIL的细胞毒性具有时间依赖性，并且可能取决于整个TIL细胞群体中Lyt-1+细胞的存在。
