Patel K R, Mayberry-Carson K J, Smith P F
J Bacteriol. 1978 Feb;133(2):925-31. doi: 10.1128/jb.133.2.925-931.1978.
Spiroplasma citri was examined by electron microscopy for morphological changes when maintained under a variety of conditions. PPLO serum fraction maintained spiral and helical morphology of S. citri at pH values of 8.0, 7.5, and 7.0, but only partially at pH 6.0 and 5.0. The absence of PPLO serum fraction resulted in round, deteriorated cells at all pH values tested. Bovine serum albumin (BSA), Phytone, soluble starch, potato starch, spermine, lipid-extracted PPLO serum fraction, and lipid-extracted BSA could substitute for PPLO serum fraction in maintaining spiral and helical morphology at pH 7.5. At pH 5.0, only BSA, lipid-extracted BSA, and lipid-extracted PPLO serum fraction were effective. Only BSA supported growth of S. citri for more than two transfers, whereas all other substitutes could not support growth longer than two transfers.
在多种条件下培养时，利用电子显微镜检查了柑橘螺原体的形态变化。PPLO血清成分在pH值为8.0、7.5和7.0时能保持柑橘螺原体的螺旋形态，但在pH 6.0和5.0时只能部分保持。在所有测试的pH值下，缺少PPLO血清成分都会导致细胞变圆并恶化。牛血清白蛋白（BSA）、植物蛋白胨、可溶性淀粉、马铃薯淀粉、精胺、脱脂PPLO血清成分和脱脂BSA在pH 7.5时可替代PPLO血清成分来维持螺旋形态。在pH 5.0时，只有BSA、脱脂BSA和脱脂PPLO血清成分有效。只有BSA能支持柑橘螺原体传代培养超过两次，而所有其他替代物都不能支持传代培养超过两次。
