绵羊脑芳基硫酸酯酶A的纯化、性质及糖蛋白特性

Purification, properties and glycoprotein nature of arylsulfatase A from sheep brain.

作者信息

Balasubramanian K A, Bachhawat B K

出版信息

Biochim Biophys Acta. 1975 Sep 22;403(1):113-21. doi: 10.1016/0005-2744(75)90014-5.

Abstract

A simple and rapid method for the purification of arylsulfatase A (EC 3.1.6.1) from sheep brain has been developed. This includes the concanavalin A-Sepharose affinity chromatography and the pH-dependent polymerization and depolymerization of the enzyme. By these methods a homogeneous enzyme was obtained and the enzyme was purified 7180-fold. Sheep brain arylsulfatase A has been shown to be a glycoprotein containing 25% neutral sugar and 0.5% sialic acid. The constituent neutral sugars were identified as glucose and mannose.

摘要

已开发出一种从羊脑中纯化芳基硫酸酯酶A（EC 3.1.6.1）的简单快速方法。该方法包括伴刀豆球蛋白A-琼脂糖亲和色谱法以及该酶的pH依赖性聚合和解聚。通过这些方法获得了一种均一的酶，该酶被纯化了7180倍。已证明羊脑芳基硫酸酯酶A是一种糖蛋白，含有25%的中性糖和0.5%的唾液酸。组成性中性糖被鉴定为葡萄糖和甘露糖。