bglY基因的突变会增加大肠杆菌K-12中自发缺失的频率。
Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12.
作者信息
Lejeune P, Danchin A
机构信息
Unité Régulation de l'Expression Génétique, Institut Pasteur, Paris, France.
出版信息
Proc Natl Acad Sci U S A. 1990 Jan;87(1):360-3. doi: 10.1073/pnas.87.1.360.
Abstract
A strong mutator effect has been observed in Escherichia coli K-12 strains mutated in the bglY gene (27 min). The frequency of point mutations is not modified in bglY mutant strains. In contrast, a strong increase in spontaneous generation of large deletions has been observed in these strains, both for chromosomal markers (10-fold increase of galETK-chlA deletions and 100-fold increase of ptsI-cysK deletions) and for plasmid DNA (100-fold increase of large deletions in the region located upstream of the chloramphenicol-resistance gene in plasmid pGR71). bglY mutations are recessive and can be complemented by a DNA fragment of 900 base pairs assumed to contain the entire bglY wild-type gene. This mutator effect is recA-independent.
摘要
在bglY基因（27分钟处）发生突变的大肠杆菌K-12菌株中观察到了强烈的诱变效应。bglY突变菌株中的点突变频率未发生改变。相反，在这些菌株中，无论是染色体标记（galETK-chlA缺失增加10倍，ptsI-cysK缺失增加100倍）还是质粒DNA（质粒pGR71中氯霉素抗性基因上游区域的大缺失增加100倍），自发产生的大缺失都有显著增加。bglY突变是隐性的，可被一个假定包含整个bglY野生型基因的900个碱基对的DNA片段互补。这种诱变效应不依赖于recA。
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