Department of Chemistry, Graduate School of Sciences and Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa, Hachioji-shi, Tokyo 192-0397, Japan.
J Chromatogr A. 2013 Oct 18;1312:87-92. doi: 10.1016/j.chroma.2013.09.021. Epub 2013 Sep 9.
The ability of denaturing ion-paired reversed phase LC to separate RNA was assessed using macro-porous polystyrene-divinylbenzene resins as the stationary phase. Using the three stationary phases with different pore size and a mobile phase containing phosphate, we separated RNAs of 20-8000 nucleotides with extremely high sensitivity, e.g., 50pg for an RNA 20 nucleotides in length, S/N=5. The method was used to separate non-coding RNAs obtained from biological sources and is suited for use with direct MS-based chemical characterization.
使用大孔聚苯乙烯-二乙烯基苯树脂作为固定相，评估了变性离子对反相 LC 分离 RNA 的能力。使用三种不同孔径的固定相和含有磷酸盐的流动相，我们分离了 20-8000 个核苷酸的 RNA，具有极高的灵敏度，例如，长度为 20 个核苷酸的 RNA 的 50pg，S/N=5。该方法用于分离来自生物来源的非编码 RNA，适合与直接基于 MS 的化学特性分析一起使用。
