Key Laboratory of Groundwater Circulation and Evolution, China University of Geosciences Beijing, Ministry of Education, Beijing 100083, China; School of Water Resources and Environment, China University of Geosciences Beijing, Beijing 100083, China.
Bioresour Technol. 2013 Nov;148:121-7. doi: 10.1016/j.biortech.2013.08.146. Epub 2013 Sep 3.
A heterotrophic/biofilm-electrode autotrophic denitrification reactor (HAD-BER) was developed to improve denitrification efficiency and reduce the consumption of organic carbon source. Maximum nitrate removal efficiency of 99.9% was gained under the optimum current density of 200 mA/m(2). The number of heterotrophic denitrification bacteria (HDB) 2.0 × 10(5) and hydrogen autotrophic denitrification bacteria (ADB) 2.0 × 10(3) in per milliliter biofilm solution were observed by the most probable number (MPN) culture, demonstrating that HDB and ADB coexist in the HAD-BER. The inorganic carbon source for autotrophic denitrification was supplied by the dissolved carbon dioxide (CO2) evolved from the heterotrophic denitrification process, indicating that there was synergistic interaction between the HDB and ADB, i.e., the organic carbon source used for denitrification could be decreased in the HAD-BER. Therefore, the developed HAD-BER would be a promising approach for nitrate removal from groundwater.
开发了一种异养/生物膜电极自养反硝化反应器 (HAD-BER)，以提高反硝化效率并减少有机碳源的消耗。在最佳电流密度 200 mA/m² 下，硝酸盐去除效率达到 99.9%。通过最可能数（MPN）培养观察到每毫升生物膜溶液中的异养反硝化细菌（HDB）和氢自养反硝化细菌（ADB）数量分别为 2.0×10(5)和 2.0×10(3)，表明 HAD-BER 中存在 HDB 和 ADB 共存。自养反硝化的无机碳源由异养反硝化过程中释放的溶解二氧化碳（CO2）提供，表明 HDB 和 ADB 之间存在协同作用，即 HAD-BER 中可减少用于反硝化的有机碳源。因此，开发的 HAD-BER 将是从地下水去除硝酸盐的一种很有前途的方法。
