Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8551, Japan.
Gastric Cancer. 2014;17(3):412-22. doi: 10.1007/s10120-013-0302-6. Epub 2013 Sep 19.
For several types of cancer, including gastric cancer (GC), tumor cells at the invasive front are considered to have a more aggressive behavior compared with those in the more central region. The aim of the present study was to analyze the expression of MMP-7, laminin γ2 and EGFR in a large number of GCs and to investigate how these expression patterns correlate with clinicopathologic parameters, infiltrative patterns, histology or mucin phenotype.
We immunohistochemically examined the expression of MMP-7, laminin γ2 and EGFR using a tissue microarray analysis of 790 GCs, and evaluated their clinicopathological significance.
MMP-7, cytoplasmic laminin γ2, extracellular laminin γ2 and EGFR expression were observed in 25, 25, 8 and 21 % of the 790 GC cases, respectively. Expression of MMP-7, cytoplasmic laminin γ2 and EGFR was associated with advanced T grade, N grade and tumor stage. Extracellular laminin γ2 expression was not associated with any clinicopathologic parameters, infiltrative patterns, histology or mucin phenotype. Furthermore, we investigated the correlations of MMP-7, laminin γ2 and EGFR expression. MMP-7 expression was significantly more frequent in positive expression of cytoplasmic laminin γ2 than negative cases, and EGFR expression was significantly more frequent in positive expression of cytoplasmic laminin γ2 and MMP-7.
Molecular expression of MMP-7, laminin γ2 or EGFR, and their combinations, may be associated with GC tumor aggressiveness. Assessment of expression of these molecules at the invasive front of primary tumors is clinically significant in predicting the malignant behavior of GC.
对于多种癌症，包括胃癌（GC），侵袭前沿的肿瘤细胞被认为比中心区域的肿瘤细胞具有更具侵袭性的行为。本研究的目的是分析大量 GC 中 MMP-7、层粘连蛋白 γ2 和 EGFR 的表达，并研究这些表达模式与临床病理参数、浸润模式、组织学或粘蛋白表型的关系。
我们使用 790 例 GC 的组织微阵列分析免疫组化检测 MMP-7、层粘连蛋白 γ2 和 EGFR 的表达，并评估其临床病理意义。
在 790 例 GC 病例中，分别观察到 MMP-7、细胞质层粘连蛋白 γ2、细胞外层粘连蛋白 γ2 和 EGFR 的表达为 25%、25%、8%和 21%。MMP-7、细胞质层粘连蛋白 γ2 和 EGFR 的表达与晚期 T 分级、N 分级和肿瘤分期相关。细胞外层粘连蛋白 γ2 的表达与任何临床病理参数、浸润模式、组织学或粘蛋白表型均无相关性。此外，我们还研究了 MMP-7、层粘连蛋白 γ2 和 EGFR 表达之间的相关性。与细胞质层粘连蛋白 γ2 阴性病例相比，MMP-7 表达在细胞质层粘连蛋白 γ2 阳性病例中更为常见，而 EGFR 表达在细胞质层粘连蛋白 γ2 和 MMP-7 阳性病例中更为常见。
MMP-7、层粘连蛋白 γ2 或 EGFR 的分子表达及其组合可能与 GC 肿瘤侵袭性有关。评估这些分子在原发性肿瘤侵袭前沿的表达对于预测 GC 的恶性行为具有临床意义。
