Perruchoud Benjamin, Egger Boris
Zoology Unit, Department of Biology, University of Fribourg, Fribourg, Switzerland.
Methods Mol Biol. 2014;1082:71-8. doi: 10.1007/978-1-62703-655-9_5.
The Drosophila visual system is an excellent model system to study the switch from proliferating to differentiating neural stem cells. In the developing larval optic lobe, symmetrically dividing neuroepithelial cells transform to asymmetrically dividing neuroblasts in a highly ordered and sequential manner. This chapter presents a protocol to visualize neural stem cell types in the Drosophila optic lobe by fluorescence confocal microscopy. A main focus is given on how to dissect, fix, immunolabel, and mount brains to reveal cellular morphology during early larval brain development.
果蝇视觉系统是研究神经干细胞从增殖状态转变为分化状态的优秀模型系统。在发育中的幼虫视叶中，对称分裂的神经上皮细胞以高度有序且连续的方式转变为不对称分裂的神经母细胞。本章介绍了一种通过荧光共聚焦显微镜观察果蝇视叶中神经干细胞类型的方法。主要重点在于如何解剖、固定、免疫标记和装片大脑，以揭示幼虫早期大脑发育过程中的细胞形态。
