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固定化黄曲霉K-03角质酶用于降解羽毛角蛋白

Immobilization of Keratinase from Aspergillus flavus K-03 for Degradation of Feather Keratin.

作者信息

Kim Jeong-Dong

机构信息

Department of Life Science, Hanyang University, Seoul 133-791, Korea.

出版信息

Mycobiology. 2005 Jun;33(2):121-3. doi: 10.4489/MYCO.2005.33.2.121. Epub 2005 Jun 30.

DOI:10.4489/MYCO.2005.33.2.121
PMID:24049486
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3774865/
Abstract

Extracellular keratinase isolated from Aspergillus flavus K-03 was immobilized on calcium alginate. The properties and reaction activities of free and immobilized keratinase with calcium alginate were characterized. The immobilized keratinase showed proteolytic activity against soluble azo-casein and azo-keratin, and insoluble feather keratin. Heat stability and pH tolerance of keratinase were greatly enhanced by immobilization. It also displayed a higher level of heat stability and an increased tolerance toward alkaline pHs compared with free keratinase. During the durability test at 40℃, 48% of the original enzyme activity of the immobilized keratinase was remained after 7 days of incubation. The immobilized keratinase exhibited better stability, thus increasing its potential for use in industrial application.

摘要

从黄曲霉K-03中分离出的胞外角蛋白酶被固定在海藻酸钙上。对游离角蛋白酶和固定在海藻酸钙上的角蛋白酶的性质及反应活性进行了表征。固定化角蛋白酶对可溶性偶氮酪蛋白、偶氮角蛋白和不溶性羽毛角蛋白具有蛋白水解活性。通过固定化，角蛋白酶的热稳定性和pH耐受性大大提高。与游离角蛋白酶相比，它还表现出更高的热稳定性和对碱性pH值的耐受性增强。在40℃的耐久性测试中，固定化角蛋白酶孵育7天后仍保留48%的原始酶活性。固定化角蛋白酶表现出更好的稳定性，因此增加了其在工业应用中的使用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/6c64aff5a95e/mb-33-121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/cd5a6208a176/mb-33-121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/19faabe71537/mb-33-121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/6c64aff5a95e/mb-33-121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/cd5a6208a176/mb-33-121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/19faabe71537/mb-33-121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b45/3774865/6c64aff5a95e/mb-33-121-g003.jpg

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本文引用的文献

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Analysis and optimization of methods using water-soluble carbodiimide for immobilization of biochemicals to porous glass.使用水溶性碳二亚胺将生物化学物质固定到多孔玻璃上的方法的分析与优化。
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