百草枯通过Notch信号通路参与人类神经干细胞的分化。

[Paraquat involves differentiation of human neural stem cells via Notch signaling].

作者信息

Chang Xiu-Li, Lu Wen, Dou Ting-ting, Sun Xia, Zhou Zhi-jun

机构信息

School of Public Health/MOE Key Lab for Public Health Safety, Fudan University, Shanghai 200032, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Jul;31(7):492-5.

PMID:24053911

Abstract

OBJECTIVE

To investigate effects of paraquat on the mRNA expression of key elements of Notch signaling (Notch1, Jagged1 and DTX1) during differentiation process of human neural stem cells (hNSCs).

METHODS

hNSCs exposed to PQ at the concentrations 0.10, 1.00, 10.00 M. Cell proliferation ability was assessed using MTT assay and mRNA expressions of Notch1, Jagged1 and DTX1 were detected by Real-time RT-PCR at 2, 4, 8, 12 d of differentiation.

RESULTS

Compared with control group, NOTCH1, JAG1 mRNA expression levels exposed to PQ at the concentration of 0.10 M significantly reduced at 2, 4, 8 d and significantly went up at 12d (P < 0.01). Compared with control group, NOTCH1, JAG1 and DTX1 mRNA expression levels exposed to PQ at the concentration of 10.00 M significantly reduced at 2, 8, 12 d (P < 0.01). PQ could down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the early stage of differentiation, then up-regulate Notch1 mRNA expression, and down-regulate Notch1, Jagged1 and DTX1 mRNA expressions at the end of differentiation.

CONCLUSION

Notch signaling pathway may be involved in differentiation of neural stem cell exposed to PQ.

摘要

目的

研究百草枯对人神经干细胞（hNSCs）分化过程中Notch信号通路关键元件（Notch1、Jagged1和DTX1）mRNA表达的影响。

方法

将hNSCs暴露于浓度为0.10、1.00、10.00 μM的百草枯中。采用MTT法评估细胞增殖能力，并在分化的第2、4、8、12天通过实时RT-PCR检测Notch1、Jagged1和DTX1的mRNA表达。

结果

与对照组相比，浓度为0.10 μM的百草枯处理组在第2、4、8天NOTCH1、JAG1 mRNA表达水平显著降低，在第12天显著升高（P < 0.01）。与对照组相比，浓度为10.00 μM的百草枯处理组在第2、8、12天NOTCH1、JAG1和DTX1 mRNA表达水平显著降低（P < 0.01）。百草枯在分化早期可下调Notch1、Jagged1和DTX1 mRNA表达，随后上调Notch1 mRNA表达，并在分化末期下调Notch1、Jagged1和DTX1 mRNA表达。