Guo Zhan-Peng, Huang Mi-Na, Liu An-Qi, Yuan Ya-Jiang, Zhao Jian-Bo, Mei Xi-Fan
Institute of Orthopedics, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China.
College of Nursing, Liaoning Medical University, Jinzhou, Liaoning Province, China.
Neural Regen Res. 2015 Aug;10(8):1321-3. doi: 10.4103/1673-5374.162767.
Expression of genes in the Notch signaling pathway is altered in the injured spinal cord, which indicates that Notch participates in repair after spinal cord injury. Buyang Huanwu decoction, a traditional Chinese herbal preparation, can promote the growth of nerve cells and nerve fibers; however, it is unclear whether Buyang Huanwu decoction affects the Notch signaling pathway in injured spinal cord. In this study, a rat model was established by injuring the T10 spinal cord. At 2 days after injury, rats were intragastrically administered 2 mL of 0.8 g/mL Buyang Huanwu decoction daily until sacrifice. Real-time reverse transcription polymerase chain reaction analysis demonstrated that at 7, 14 and 28 days after injury, the expression of Notch1 was increased in the Buyang Huanwu decoction group compared with controls. These findings confirm that Buyang Huanwu decoction can promote the expression of Notch1 in rats with incomplete spinal cord injury, and may indicate a mechanism to promote the repair of spinal cord injury.
Notch信号通路中的基因在脊髓损伤后表达发生改变，这表明Notch参与脊髓损伤后的修复。补阳还五汤是一种传统的中药制剂，可促进神经细胞和神经纤维的生长；然而，补阳还五汤是否影响脊髓损伤后的Notch信号通路尚不清楚。在本研究中，通过损伤T10脊髓建立大鼠模型。损伤后2天，大鼠每天灌胃2 mL 0.8 g/mL补阳还五汤，直至处死。实时逆转录聚合酶链反应分析表明，损伤后7、14和28天，补阳还五汤组Notch1的表达高于对照组。这些发现证实补阳还五汤可促进不完全性脊髓损伤大鼠Notch1的表达，并可能提示其促进脊髓损伤修复的机制。
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