Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, Edificio Marie Curie (Anexo), Campus Universitario de Rabanales, E-14071 Córdoba, Spain.
Food Chem. 2014 Jan 15;143:341-7. doi: 10.1016/j.foodchem.2013.07.136. Epub 2013 Aug 7.
An organic solvent-free microextraction was proposed as a simple and fast sample treatment for the determination of PAH4 (viz. benz[a]anthracene, chrysene, benzo[b]fluorantene and benzo[a]pyrene). The method involved the stirring of 200mg of foodstuff with 200μL of a supramolecular solvent made up of octanoic acid/tetrabutylammonium octanoate vesicles for 10min. Then, the extract was analysed by liquid chromatography/fluorescence detection. Neither dilution nor further clean-up steps of the extracts were needed. The limit of quantitation of the method (0.3-0.7μgkg(-1)) was below the threshold limit established for benzo[a]pyrene in food by EU directives (1-10μgkg(-1)). The method was successfully applied to the analysis of smoked meat and fish, bivalve mollusks and processed cereal-based food for infants. Benzo[a]pyrene was quantified and/or detected in most of the analysed samples. The recoveries obtained for PAH4 were from 92% to 103% with relative standard deviations less than 5%.
一种无有机溶剂的微萃取方法被提出，作为一种简单快速的样品处理方法，用于测定 PAH4（即苯并[a]蒽、䓛、苯并[b]荧蒽和苯并[a]芘）。该方法包括将 200mg 食物与 200μL 由辛酸/四丁基辛酸囊泡组成的超分子溶剂在 10min 内搅拌。然后，通过液相色谱/荧光检测分析提取物。无需对提取物进行稀释或进一步净化处理。该方法的定量限（0.3-0.7μgkg(-1))低于欧盟指令规定的食品中苯并[a]芘的限量（1-10μgkg(-1))。该方法成功应用于烟熏肉和鱼、双壳贝类和婴儿用加工谷物基食品的分析。在大多数分析样品中都定量或检测到了苯并[a]芘。PAH4 的回收率在 92%至 103%之间，相对标准偏差小于 5%。
