Laboratoire de Parasitologie et Maladies Parasitaires, INP-ENVT, École Nationale Vétérinaire de Toulouse, Toulouse, France.
Appl Environ Microbiol. 2013 Dec;79(23):7439-44. doi: 10.1128/AEM.02598-13. Epub 2013 Sep 20.
Bartonella henselae (Rhizobiales: Bartonellaceae) is a Gram-negative fastidious bacterium of veterinary and zoonotic importance. The cat flea Ctenocephalides felis (Siphonaptera: Pulicidae) is the main recognized vector of B. henselae, and transmission among cats and humans occurs mainly through infected flea feces. The present study documents the use of a quantitative molecular approach to follow the daily kinetics of B. henselae within the cat flea and its excreted feces after exposure to infected blood for 48 h in an artificial membrane system. B. henselae DNA was detected in both fleas and feces for the entire life span of the fleas (i.e., 12 days) starting from 24 h after initiation of the blood meal.
汉赛巴尔通体(Rhizobiales:Bartonellaceae 科)是一种具有兽医和人畜共患重要性的革兰氏阴性需氧细菌。猫栉首蚤(Ctenocephalides felis)(Siphonaptera:Pulicidae 科)是汉赛巴尔通体的主要公认传播媒介,猫和人类之间的传播主要通过受感染的跳蚤粪便进行。本研究记录了一种定量分子方法的使用,该方法用于在人工膜系统中暴露于感染血液 48 小时后,在猫蚤及其排泄的粪便中,在整个跳蚤寿命(即,12 天)内,跟踪汉赛巴尔通体的日动力学。从开始吸血后 24 小时起,在整个跳蚤寿命(即 12 天)内,都可以在跳蚤和粪便中检测到汉赛巴尔通体 DNA。