Department of Urology, Faculty of Medicine, Dumlupinar University, 43270, Kutahya, Turkey,
Mol Biol Rep. 2013 Oct;40(10):5733-40. doi: 10.1007/s11033-013-2676-2. Epub 2013 Sep 22.
The aim of the present study was to evaluate the protective effects of the NF-кB inhibition with pyrrolidine-dithiocarbamate (PDTC) in ischemia-reperfusion (I/R) injury in the rat bladder. Twenty-four Sprague-Dawley male rats were divided into three groups. Group I; (n = 8) control, group II; (n = 8) I/R group; group III (n = 8) I/R and PDTC treatment. Superoxide dismutase (SOD), catalase (CAT), and gluatathione-S-transferase (GST) enzymes was studied in bladder tissue. Lipid peroxidation (as TBARS) levels in tissue homogenate were measured with thiobarbituric acid reaction. All the slides were stained with NF-кB, p53 and HSP60 immunohistochemistry for detection genome destruction and tissue stress, respectively. Our results show that the mean TBARS levels were significantly higher in group II (p < 0.05). The TBARS levels were significantly decreased in group III compared with the group II (p < 0.05). CAT, SOD and GST activities were decreased in group II, but these enzymes levels were significantly increased in group III according to the group II (p < 0.05). Under microscopic evaluation NF-кB expression increased significantly in group II compared to the group I (p < 0.05) and then decreased in group III (p < 0.05). HSP60 and p53 expression in group II was increased significantly compared with group I. Under microscopic evaluation we detected that HSP60 and p53 expression was increased significantly in group II compared with group I. In group III PDTC administration was decreased the HSP60 and p53 expression, this difference was statistically significant (p < 0.05). The results of the present study have demonstrated that NF-кB inhibition with PDTC protects and provides beneficial effects on ischemia/reperfusion stress related bladder tissue destruction.
本研究旨在评估核因子-κB（NF-κB）抑制吡咯烷二硫代氨基甲酸盐（PDTC）对大鼠膀胱缺血再灌注（I/R）损伤的保护作用。24 只雄性 Sprague-Dawley 大鼠分为三组。I 组（n = 8）为对照组，II 组（n = 8）为 I/R 组，III 组（n = 8）为 I/R 和 PDTC 治疗组。研究了膀胱组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽-S-转移酶（GST）的活性。用硫代巴比妥酸反应法测定组织匀浆中脂质过氧化（TBARS）水平。所有切片均用 NF-κB、p53 和 HSP60 免疫组化染色，分别检测基因组破坏和组织应激。结果显示，II 组 TBARS 水平显著升高（p < 0.05）。与 II 组相比，III 组 TBARS 水平显著降低（p < 0.05）。与 II 组相比，II 组 CAT、SOD 和 GST 活性降低，但 III 组这些酶水平显著升高（p < 0.05）。在显微镜下评估时，与 I 组相比，II 组 NF-κB 表达显著增加（p < 0.05），然后在 III 组中减少（p < 0.05）。与 I 组相比，II 组 HSP60 和 p53 表达显著增加。在显微镜下评估时，我们发现与 I 组相比，II 组 HSP60 和 p53 表达显著增加。在 III 组中，PDTC 给药降低了 HSP60 和 p53 的表达，这种差异具有统计学意义（p < 0.05）。本研究结果表明，NF-κB 抑制吡咯烷二硫代氨基甲酸盐对缺血再灌注相关膀胱组织损伤具有保护作用并提供有益影响。
