拟南芥发育的实时成像。

Live imaging of Arabidopsis development.

作者信息

von Wangenheim Daniel, Daum Gabor, Lohmann Jan U, Stelzer Ernst K, Maizel Alexis

机构信息

Physical Biology, Frankfurt Institute for Molecular Life Sciences (FMLS), Goethe Universität Frankfurt am Main, Frankfurt am Main, Germany.

出版信息

Methods Mol Biol. 2014;1062:539-50. doi: 10.1007/978-1-62703-580-4_28.

DOI:10.1007/978-1-62703-580-4_28

PMID:24057385

Abstract

Live cell imaging is an essential methodology for studying the structure, dynamics, and functions of cells in a living plant under normal or stressed growth conditions. Arabidopsis thaliana is perfectly amenable to various live microscopy techniques. In this chapter, we provide guidelines to design live-imaging experiments. We discuss specifically the respective advantage of each microscopy technique, the choice of reporter, and the preparation of the sample. Detailed protocols for imaging of shoot and roots are provided.

摘要

活细胞成像对于研究正常或胁迫生长条件下活植物中细胞的结构、动态和功能而言是一种必不可少的方法。拟南芥非常适合各种活细胞显微镜技术。在本章中，我们提供了设计活细胞成像实验的指南。我们具体讨论了每种显微镜技术的各自优势、报告基因的选择以及样品的制备。还提供了茎和根成像的详细方案。