Department of Pediatrics, Faculty of Medical, Dokuz Eylul University , Izmir , Turkey .
Ren Fail. 2014 Feb;36(1):55-7. doi: 10.3109/0886022X.2013.832863. Epub 2013 Sep 24.
High serum bilirubin is antioxidant and cytoprotective. We evaluated if urine samples of hyperbilirubinemic newborns impede uropathogenic Escherichia coli growth. Bag-urine samples of hyperbilirubinemic newborns (study group) were cultured at presentation and during remission. Urine sample were obtained only once from healthy newborns (control group). Escherichia coli [2 × 104 colony-forming unit (cfu)/mL] was inoculated into the sterile urine samples and colony counts were determined after 24 h. Bilirubin levels at presentation and remission were also recorded. Escherichia coli colony counts of the control versus study groups and of the presentation versus remission samples in the study group were compared. There were 13 study and 17 control cases. Escherichia coli colony counts were not different in the study group at presentation versus remission (5.4 ± 0.7 vs. 5.5 ± 0.8 log10, respectively; p = 0.659). Escherichia coli colony count of the control group (5.2 ± 0.6 log10) was also not different from the study group. In conclusion, the urine of hyperbilirubinemic newborns did not affect the growth rate of uropathogenic E. coli.
血清胆红素水平升高具有抗氧化和细胞保护作用。我们评估了高胆红素血症新生儿的尿液样本是否会抑制尿路致病性大肠杆菌的生长。高胆红素血症新生儿（研究组）在出现时和缓解时采集袋装尿液样本进行培养。仅从健康新生儿（对照组）中采集一次尿液样本。将大肠杆菌[2×104 个菌落形成单位（cfu）/mL]接种到无菌尿液样本中，并在 24 小时后测定菌落计数。同时记录出现时和缓解时的胆红素水平。比较了对照组和研究组的大肠杆菌菌落计数以及研究组出现时和缓解时样本的大肠杆菌菌落计数。研究组有 13 例和对照组有 17 例。研究组中，出现时与缓解时的大肠杆菌菌落计数没有差异（分别为 5.4±0.7 和 5.5±0.8 log10；p=0.659）。对照组的大肠杆菌菌落计数（5.2±0.6 log10）也与研究组无差异。总之，高胆红素血症新生儿的尿液不会影响尿路致病性大肠杆菌的生长速度。
