Zheng Hong-yan, Shi Yan-hong, Zhang Li-fang, Chen Ying-zhun
Department of Pathology, the Second Affiliated Clinical Hospital of Harbin Medical University, Harbin 150086, China.
Zhonghua Bing Li Xue Za Zhi. 2013 Jun;42(6):372-5. doi: 10.3760/cma.j.issn.0529-5807.2013.06.004.
To investigate the expression of endothelium tight junction protein Claudin-5 and intercellular adhesion molecule CD99 in solid-pseudopapillary neoplasms (SPN) and neuroendocrine tumors of pancreas (P-NET), and their significance in the differential diagnoses.
Immunohistochemical staining of Claudin-5 and CD99 was performed in 37 cases SPN and 21 cases of P-NET.
Membranous Claudin-5 expression was observed in all cases of SPN but was absent in all cases of P-NET. The difference was significant (P < 0.01). In SPN, 91.9% (34/37) of the cases displayed paranuclear dot-like immunoreactivity for CD99; in contrast, 61.9% (13/21) of the cases of P-NET displayed membranous staining (P < 0.01). There was a positive association between the expression of Claudin-5 and CD99 in SPN (r = 0.421,P = 0.001).
Although the macroscopic and microscopic features of SPN are quite characteristic, they may not allow confident differentiation from P-NET in all cases, especially when these characteristics are not classical. If necessary, immunostaining for Claudin-5 and CD99 can help to differentiate between these entities.
探讨紧密连接蛋白Claudin-5及细胞间黏附分子CD99在胰腺实性假乳头状肿瘤（SPN）和胰腺神经内分泌肿瘤（P-NET）中的表达情况及其在鉴别诊断中的意义。
对37例SPN和21例P-NET进行Claudin-5和CD99免疫组织化学染色。
所有SPN病例均可见Claudin-5膜表达，而所有P-NET病例均无Claudin-5膜表达。差异有统计学意义（P<0.01）。在SPN中，91.9%（34/37）的病例CD99呈核旁点状免疫反应；相比之下，61.9%（13/21）的P-NET病例CD99呈膜染色（P<0.01）。SPN中Claudin-5和CD99的表达呈正相关（r=0.421，P=0.001）。
尽管SPN的大体及镜下特征颇具特点，但在所有病例中可能无法与P-NET进行可靠鉴别，尤其是当这些特征不典型时。必要时，Claudin-5和CD99免疫染色有助于鉴别这两种病变。
