Laboratory of Physical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
J Chromatogr A. 2013 Nov 1;1314:138-41. doi: 10.1016/j.chroma.2013.09.032. Epub 2013 Sep 13.
This work provides a theoretical basis for an empirical model proposed in Fasoula et al. (2013) [2] for the retention of monoprotic acids/bases during linear organic modifier gradient runs performed with a fixed change of organic content but for different gradient durations and in different eluent pHs. Based on the analytical expressions derived in the above publication for the retention of monoprotic solutes under organic modifier gradients in different mobile phase pHs it was proved that the dependences of retention time of all monoprotic acids/bases upon each of two factors governed the gradient elution under consideration are always the following: practically linear upon gradient duration (for constant pH) and sigmoid upon pH (for constant gradient duration).
这项工作为 Fasoula 等人（2013）[2]提出的经验模型提供了理论依据，该模型用于保留在不同有机含量变化、不同梯度时间和不同洗脱液 pH 值下进行的线性有机改性剂梯度运行期间的一元酸/碱。基于上述出版物中在不同流动相 pH 值下的有机改性剂梯度中一元溶质保留的分析表达式，证明了所有一元酸/碱的保留时间对两个控制所考虑梯度洗脱的因素中的每一个的依赖关系始终如下：在（恒定 pH 值下）几乎呈线性，在 pH 值（在恒定梯度时间下）呈 S 型。
