Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, Rutgers University, The State University of New Jersey, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
Environ Pollut. 2014 Jan;184:222-30. doi: 10.1016/j.envpol.2013.08.019. Epub 2013 Sep 21.
Anaerobic enrichment cultures derived from contaminated Kymijoki River sediments dechlorinated 1,2,3,4-tetrachlorodibenzofuran (1,2,3,4-tetra-CDF), octachlorodibenzofuran (octa-CDF) and 1,2,3,4-tetrachlorodibenzo-p-dioxin (1,2,3,4-tetra-CDD). 1,2,3,4-tetra-CDF was dechlorinated via 1,2,3-, 2,3,4-, and 1,3,4/1,2,4-tri-CDFs to 1,3-, 2,3-, and 2,4-di-CDFs and finally to 4-mono-CDF. The dechlorination rate of 1,2,3,4-tetra-CDF was generally slower than that of 1,2,3,4-tetra-CDD. The rate and extent of 1,2,3,4-tetra-CDD dechlorination was enhanced by addition of pentachloronitrobenzene (PCNB) as a co-substrate. Dechlorination of spiked octa-CDF was observed with the production of hepta-, hexa-, penta- and tetra-CDFs over 6 months. Two major phylotypes of the Chloroflexi community showed an increase, one of which was identical to the Dehalococcoides mccartyi Pinellas subgroup. A set of twelve putative reductive dehalogenase (rdh) genes increased in abundance with addition of 1,2,3,4-tetra-CDF, 1,2,3,4-tetra-CDD and/or PCNB. This information will aid in understanding how indigenous microbial communities impact the fate of PCDFs and in developing strategies for bioremediation of PCDD/F contaminated sediments.
从受污染的凯米河沉积物中获得的厌氧富集培养物脱氯了 1,2,3,4-四氯二苯并呋喃（1,2,3,4-四氯二苯并呋喃）、八氯二苯并呋喃（八氯二苯并呋喃）和 1,2,3,4-四氯二苯并对二恶英（1,2,3,4-四氯二苯并对二恶英）。1,2,3,4-四氯二苯并呋喃通过 1,2,3-、2,3,4-和 1,3,4/1,2,4-三氯二苯并呋喃脱氯为 1,3-、2,3-和 2,4-二氯二苯并呋喃，最终为 4-单氯二苯并呋喃。1,2,3,4-四氯二苯并呋喃的脱氯速率通常比 1,2,3,4-四氯二苯并对二恶英慢。添加五氯硝基苯（PCNB）作为共底物可增强 1,2,3,4-四氯二苯并对二恶英的脱氯速率和程度。在 6 个月的时间里，观察到添加的八氯二苯并呋喃脱氯，生成了庚、六、五和四氯二苯并呋喃。一个氯杆菌门群落的两个主要生物型增加，其中一个与皮尼拉斯组的 Dehalococcoides mccartyi 相同。一组 12 种假定的还原脱卤酶（rdh）基因随着 1,2,3,4-四氯二苯并呋喃、1,2,3,4-四氯二苯并对二恶英和/或 PCNB 的添加而增加。这些信息将有助于了解土著微生物群落如何影响 PCDFs 的命运，并为受 PCDD/F 污染的沉积物的生物修复开发策略。
